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目的研究喉癌中STK15基因扩增、mRNA和蛋白表达增高在喉癌发生、发展中的作用。方法应用差异PCR方法检测40例喉鳞状细胞癌及癌旁正常对照组织STK15基因扩增的情况;应用逆转录PCR方法检测同批标本STK15mRNA表达情况;用免疫组织化学方法分析STK15蛋白表达。结果肿瘤组织中STK15基因扩增率为35%(14/40);STK15mRNA表达增高占67.5%(27/40);STK15蛋白表达的阳性率为72.5%(29/40);将扩增与表达的结果与喉癌患者的临床各项指标进行统计学分析,STK15基因扩增、STK15mRNA表达增高与肿瘤的分化程度显著相关(P<0.05);STK15蛋白表达与肿瘤的分化程度和病理分级显著相关(P<0.05)。结论喉癌中STK15基因扩增、mRNA和蛋白表达水平增高,导致中心体复制异常、染色体不稳定,可能在喉癌的发生及恶性进展中起一定作用。
Objective To study the role of STK15 gene amplification, mRNA and protein expression in the development and progression of laryngeal carcinoma in laryngeal cancer. Methods Differential PCR was used to detect the amplification of STK15 gene in 40 cases of laryngeal squamous cell carcinoma and adjacent normal tissues. Reverse transcription PCR was used to detect the expression of STK15 mRNA in the same samples. The expression of STK15 protein was analyzed by immunohistochemistry. Results The rate of STK15 gene amplification was 35% (14/40) in tumor tissues, 67.5% (27/40) in STK15 and 72.5% (29/40) in STK15. The amplification and expression of STK15 (P <0.05). The expression of STK15 protein was significantly correlated with the degree of tumor differentiation and pathological grade (P <0.05). Conclusion STK15 gene amplification, mRNA and protein expression are increased in laryngeal carcinoma, resulting in abnormal replication of centrosome and chromosomal instability, which may play a role in the occurrence and malignant progression of laryngeal carcinoma.