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目的研究铜绿假单胞菌制剂(pseudomonas aeruginosa with mannose sensitive hemagglutination pili,PAMSHA)由甘露糖依赖方式影响Bad、Bax及Bcl-2的表达进而影响癌细胞的增殖和细胞周期进程,为治疗宫颈癌的新的生物制剂提供依据。方法采用单独处理PA-MSHA或甘露糖与PA-MSHA联合处理宫颈癌Hela细胞,随后采用MTT法检测细胞增殖,流式检测细胞周期,荧光定量PCR和western blot检测凋亡抑制蛋白Bcl-2和促凋亡蛋白Bad、Bax的表达变化。结果 PA-MSHA单独处理宫颈癌Hela后细胞增殖减慢,细胞周期阻滞在G0/G1期,同时荧光定量PCR和western blot检测显示促凋亡蛋白Bad、Bax的表达增加而凋亡抑制蛋白Bcl-2表达减少。而甘露糖与PA-MSHA共培育组与空白组对比在细胞增殖、细胞周期及凋亡蛋白表达上差异无统计学意义。结论 PA-MSHA可以影响Bad、Bax及Bcl-2的表达进而影响癌细胞的增殖和细胞周期进程,且这种作用为甘露糖敏感型,该结果为其成为治疗宫颈癌的新的生物制剂提供了可靠依据。
Objective To study the effect of mannose-sensitive hemagglutination pili (PAMSHA) on the expression of Bad, Bax and Bcl-2 in mannose-dependent hematopoietic pili (PAMSHA) cells and to influence the proliferation and cell cycle progression of cancer cells. New biological agents provide the basis. Methods Hela cells were treated with PA-MSHA or mannose alone and PA-MSHA. Cell proliferation was detected by MTT assay. Cell cycle was detected by flow cytometry. Fluorescence quantitative PCR and western blot were used to detect the expression of Bcl-2 and The expression of pro-apoptotic proteins Bad and Bax. Results The proliferation of Hela cells treated with PA-MSHA alone was slowed down and cell cycle arrest was at G0 / G1 phase. Fluorescence quantitative PCR and western blot showed that the expression of the pro-apoptotic proteins Bad and Bax increased while that of the apoptosis-inhibitory protein Bcl -2 expression decreased. Mannose and PA-MSHA co-cultivation group compared with the blank group in cell proliferation, cell cycle and apoptosis protein expression difference was not statistically significant. Conclusions PA-MSHA can affect the expression of Bad, Bax and Bcl-2 and thus affect the proliferation and cell cycle progression of cancer cells, and this effect is mannose-sensitive and this result provides a novel biologic agent for the treatment of cervical cancer Reliable basis.