观察了Ｍ－ＣＳＦ预处理、ＩＦＮ－γ预处理、长程培养及新城疫病毒（ｎｅｗ－ｃａｓｔｌｅｄｉｓｅａｓｅｖｉｒｕｓ，ＮＤＶ）刺激４个因素对正常人外周血单核细胞体外产生ＩＦＮ－α水平的影响，采用生物活性法检测ＩＦＮ－α。结果表明：单独使用Ｍ－ＣＳＦ不能诱生ＩＦＮ－α；单独使用ＩＦＮ－γ或单独使用ＮＤＶ均只诱生较低水平ＩＦＮ－α，只有Ｍ－ＣＳＦ＋ＩＦＮ－γ＋ＮＤＶ或ＩＦＮ－γ＋ＮＤＶ联合使用方可诱生较高水平的ＩＦＮ－α，并且发现Ｍο、－Ｍφ长程培养也是向高产发展的一个重要因素。结论：人血Ｍο、－Ｍφ在适当体外培养条件下，可以诱发较高水平的ＩＦＮ－α The effects of M-CSF pretreatment, IFN-γ pretreatment, long-term culture and new-castledisease virus (NDV) stimulation on the level of IFN-α produced by normal human peripheral blood mononuclear cells were observed. IFN-α activity assay. The results showed that: M-CSF alone could not induce IFN-α; IFN-γ alone or NDV alone induced lower levels of IFN-α, only M-CSF + IFN-γ + NDV or IFN-γ + Higher levels of IFN-α were found, and Mο, -Mφ long-term culture was also found to be an important factor in high-yielding development. Conclusion: Human blood Mο, -Mφ can induce higher levels of IFN-α