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用处于对数生长期的发根农杆菌（Ｒ１０００，Ａ４和１５８３４）感染甜橙子叶外植体，与菌体共培养３天后，转至含头孢霉素的无激素ＭＴ培养基进行选择培养，１５天后开始产生毛状根，转化率在２５％～３６％之间。毛状根在分单根进行的克隆培养中能稳定地保持激素自主性生长。在无激素的ＭＴ培养基上，毛状根可自发地产生不定芽，适当添加一定种类及浓度植物生长调节剂，可明显提高毛状根再生芽的频率。再生植株叶片和毛状根提取液的电泳检测表明，甘露碱合成酶及农杆碱合成酶均呈阳性，证明Ｒｉ质粒的Ｔ—ＤＮＡ转移并整合到甜橙细胞核基因组中，在转化体及再生植株中得到了表达。
In the logarithmic growth phase Agrobacterium rhizi (R1000, A4 and 15834) infected sweet orange cotyledon explants with the bacterial co-culture for 3 days, go to cephalosporin-free hormone-free MT medium for selective culture, 15 days later Begin to produce hairy roots, the conversion rate of 25% to 36%. Hairy roots stably maintain hormone-independent growth in single root clonal cultures. In hormone-free MT medium, hairy roots spontaneously produce adventitious buds, appropriate to add certain types and concentrations of plant growth regulators, can significantly improve hairy root regeneration buds frequency. The electrophoresis analysis of the regenerated plant leaves and hairy root extract showed that both mannopine synthase and aglycone synthase were positive, which proved that the T-DNA of Ri plasmid was transferred and integrated into the nuclear genome of sweet orange. In the transformants and regeneration Plants have been expressed.