目的 :比较两种不同体外血脑屏障 (BBB)模型的功能差异。方法 :采用大鼠脑微血管内皮细胞 (BMEC)、人脐静脉内皮细胞(HUVEC)分别与大鼠脑TIA共同培养于Transwell多聚酯膜的两侧 ,建立两种体外血脑屏障模型。测定两种模型中荧光素钠、牛血清白蛋白的通透量以及屏障特异性酶γ GT的含量。结果 :BEMC构建的BBB模型对低分子量荧光素钠和大分子量蛋白的通透性都小于HUVEC组。在细胞生物化学方面 ,BMEC组γ GT含量为 ( 11 3± 0 66)mgprotein ,而HUVEC组则未测出。结论 :同种属的BMEC与TIA共培养构建的BBB模型是模拟体内BBB的理想选择。 PURPOSE: To compare the functional differences of two different models of the blood-brain barrier (BBB) ​​in vitro. Methods: Two kinds of blood-brain barrier models were established by using rat brain microvascular endothelial cells (BMECs) and human umbilical vein endothelial cells (HUVECs) co-cultured with rat brain TIA on both sides of Transwell membrane. The permeation of sodium fluorescein and bovine serum albumin and the content of barrier-specific enzyme γ-GT were measured in the two models. Results: The BBB model constructed by BEMC had less permeability to low molecular weight sodium fluorescein and higher molecular weight proteins than HUVEC. In cell biochemistry, the γ GT content in BMEC group was (11 3 ± 0 66) mgprotein, but not in HUVEC group. CONCLUSION: The BBB model co-cultured with the same species of BMECs and TIA is an ideal model to simulate BBB in vivo.