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目的:探讨预运动训练对大鼠脑梗死后缺血侧纹状体处谷氨酸(Glu)水平及其代谢型受体(mGluR1)mRNA表达的影响,探讨预运动训练对缺血性脑梗死的保护机制。方法:将Sprague-Dawley大鼠随机分为假手术组、缺血组和缺血前运动组。运动方案采用电动跑台,30mim/d,20m/min,5d/week,共2周。采用微透析技术收集各组大鼠缺血前、缺血期间(40,80和120min)和再灌注后(40,80,120,160,200和240min)的脑细胞外液,用于测定兴奋性氨基酸Glu含量的变化。同时运用RT-PCR技术半定量分析缺血80min和再灌注240min时纹状体脑组织内的mGluR1 mRNA表达水平。结果:缺血组Glu水平在缺血40、80、120min和再灌注40、120、160min时间点与缺血前比较有显著性差异(P<0.05,P<0.01)。运动组各时间点的Glu水平普遍显著低于缺血组(P<0.05,P<0.01),而缺血时各时间点显著高于假手术组(P<0.05,P<0.01)。缺血80min和再灌注240min时缺血组和运动组mGluR1 mRNA表达均显著高于假手术组,运动组显著低于缺血组(P<0.05)。结论:预运动训练对随后发生的脑梗死纹状体内重要的兴奋性氨基酸递质Glu的过度释放有一定程度的抑制作用,这种抑制作用可能与mGluR1 mRNA的下调有某种联系。
OBJECTIVE: To investigate the effect of pre-training on glutamate (Glu) level and mRNA expression of mGluR1 in ischemic striatum after cerebral infarction in rats, and to investigate the effect of pre-training on ischemic cerebral infarction Protection mechanism. Methods: Sprague-Dawley rats were randomly divided into sham operation group, ischemia group and pre-ischemic exercise group. Exercise program using electric treadmill, 30mim / d, 20m / min, 5d / week, a total of 2 weeks. The microdialysis technique was used to collect brain extracellular fluid before and after ischemia (40, 80 and 120 min) and after reperfusion (40, 80, 120, 160, 200 and 240 min) in rats to determine the changes of excitatory amino acid Glu . RT-PCR was used to semi-quantitatively analyze the expression of mGluR1 mRNA in the striatum brain tissue after 80 min of ischemia and 240 min of reperfusion. Results: The level of Glu in ischemic group was significantly different from that before ischemia at 40, 80, 120 min and 40, 120, 160 min after reperfusion (P <0.05, P <0.01). The level of Glu in exercise group at each time point was significantly lower than that in ischemic group (P <0.05, P <0.01), and at each time point in ischemia group was significantly higher than that in sham operation group (P <0.05, P <0.01). The expression of mGluR1 mRNA in ischemic group and exercise group at 80 min ischemia and 240 min reperfusion were significantly higher than those in sham operation group and exercise group (P <0.05). CONCLUSION: Pre-exercise training can inhibit the over-release of Glu which is an important excitatory amino acid in striatum in the subsequent cerebral infarction. This inhibition may be related to the down-regulation of mGluR1 mRNA.