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目的:研究美托康(杏芎氯化钠注射液)对大鼠局灶性脑缺血再灌注损伤后血液中纤维蛋白原(Fg)、血小板α颗粒膜蛋白140(GMP140)、组织型纤溶酶原激活物(tPA)表达的影响。方法:80只健康雄性SD大鼠随机分为手术后3d组(n=40)和7d组(n=40);每组再分为假手术组、缺血对照组、金纳多组和美托康组。建立大鼠局灶性脑缺血模型,采用酶联免疫吸附法发色底物法检测GMP140活性、tPA活性,凝胶空斑法检测Fg含量。结果:金纳多组Fg含量显著高于缺血对照组,且7d组也明显高于美托康组(P<0.05);金纳多组和美托康组GMP140活性显著低于缺血对照组(P<0.05),但金纳多组与美托康组无显著差异(P>0.05);缺血对照组和金纳多7d组tPA活性较假手术组显著降低(P<0.05),美托康组显著高于缺血对照组和金纳多组(P<0.05),但与假手术组无显著差异(P>0.05)。结论:美托康可降低Fg含量和GMP140活性,增高tPA活性,且在降低Fg含量和增高tPA活性方面优于金纳多,而在降低GMP140活性方面与金纳多相当。
OBJECTIVE: To study the blood fibrinogen (Fg), platelet alpha granule membrane protein 140 (GMP140), and tissue-type fibrinogen of metoprolol (Apricot Sodium Chloride Injection) after focal cerebral ischemia-reperfusion injury in rats. The effect of plasminogen activator (tPA) expression. METHODS: Eighty healthy male Sprague-Dawley rats were randomly divided into three groups (n=40) and seven days (n=40) after surgery. Each group was subdivided into sham operation group, ischemic control group, Ginaton group and metoprolol. Kang Group. The rat model of focal cerebral ischemia was established. GMP140 activity and tPA activity were detected by enzyme-linked immunosorbent assay with chromogenic substrate method, and Fg content was detected by gel plaque assay. RESULTS: The Fg content in Ginaton group was significantly higher than that in the ischemic control group, and it was also significantly higher in the 7d group than in the meitocam group (P<0.05). The GMP140 activity was significantly lower in the Ginaton group and the maitopon group than in the ischemic control group. (P<0.05), but there was no significant difference between the Ginaton group and the metoprolol group (P>0.05); the activity of tPA in the ischemic control group and the Ginaton 7d group was significantly lower than that in the sham operation group (P<0.05). The Tocong group was significantly higher than the ischemia group and the Ginaton group (P<0.05), but there was no significant difference with the sham group (P>0.05). CONCLUSION: Metoprolol can reduce Fg content and GMP140 activity, increase tPA activity, and is superior to Ginaton in reducing Fg content and increasing tPA activity, and is comparable to Ginaton in reducing GMP140 activity.