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目的:用HPLC同时测定附子中盐酸多巴胺和去甲猪毛菜碱的含量。方法:采用Agilent Eclipse C18柱(4.6 mm×250 mm,5μm),流动相为0.1%磷酸水溶液,流速1 mL.min-1,柱温35℃,检测波长280 nm。结果:盐酸多巴胺和去甲猪毛菜碱色谱峰与其他色谱峰分离良好,样品中盐酸多巴胺和去甲猪毛菜碱色谱峰的DAD光谱与对照品一致,进样量在0.00521～0.521μg和0.005 24～0.524μg线性关系良好,平均回收率分别为100.6%和96.5%,RSD分别为2.0%和1.0%(n=6)。结论:实验含量测定方法准确、快速,为附子质量控制提供参考方法。
Objective: To determine the contents of dopamine hydrochloride and norrabine in aconite by HPLC simultaneously. METHODS: Agilent Eclipse C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase was 0.1% phosphoric acid solution and the flow rate was 1 mL.min-1. The column temperature was 35 ℃ and the detection wavelength was 280 nm. Results: Dopamine hydrochloride and norratolactan peak were well separated from other chromatographic peaks. The DAD spectra of dopamine hydrochloride and norbine peak in the samples were consistent with those of the reference samples. The injection volumes ranged from 0.00521 to 0.521 μg and The linear range of 0.005 24-0.524 μg was good with average recoveries of 100.6% and 96.5%, respectively, with RSDs of 2.0% and 1.0%, respectively (n = 6). Conclusion: The method of determination of test content is accurate and rapid, which provides a reference method for the quality control of aconite.