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目的 :研究GLUT1mRNA在食管癌组织及正常食管黏膜的表达水平。方法 :对 16例食管癌病人手术标本的肿瘤组织及正常食管黏膜进行总RNA的提取 ,并进行GLUT1mRNA的RT -PCR扩增。结果 :16例肿瘤组织标本中 ,14例有GLUT1mRNA阳性表达 ;相应的 16例正常组织标本中 ,10例有GLUT1mRNA阳性表达。结论 :GLUT1mRNA在食管癌组织中的表达水平较正常食管黏膜显著升高 ,对于食管癌的早期诊断有一定的帮助。
Objective : To study the expression of GLUT1 mRNA in esophageal cancer tissues and normal esophageal mucosa. Methods: Total RNA was extracted from tumor tissue and normal esophageal mucosa of 16 surgical specimens of esophageal cancer patients. GLUT1 mRNA was amplified by RT-PCR. RESULTS: Of the 16 tumor specimens, 14 cases had positive expression of GLUT1 mRNA. Of the 16 normal tissue specimens, 10 cases had positive expression of GLUT1 mRNA. Conclusion: The expression level of GLUT1 mRNA in esophageal cancer tissue was significantly higher than that in normal esophageal mucosa, which is helpful for the early diagnosis of esophageal cancer.