将PCR扩增的淋球菌CppB膜蛋白基因区段,分别克隆在游离蛋白表达载体pKK223-3和融合蛋白表达载体pWR450-1上,以点杂交证实了克隆子。控制合适表达条件,在大肠杆菌中获高效表达,免疫印迹证实其可被淋病患者血清特异识别,将融合蛋白表达质粒转入减毒鼠伤寒沙门氏菌中也获表达,为淋病口服活疫苗的制备奠定了基础。 The PCR amplification of the gonococcal CppB membrane protein gene segments were cloned in the free protein expression vector pKK223-3 and the fusion protein expression vector pWR450-1, confirmed by dot blot hybridization clones. Controlled expression of the appropriate conditions in E. coli were highly expressed, confirmed by immunoblotting gonococcal patients can be specifically identified serum, the fusion protein expression plasmid was also transferred into attenuated Salmonella typhimurium was also prepared for oral preparation of live gonorrhea vaccine laid The foundation.