基因表达的变化是调控细胞生命活动的核心机制 ,分离并克隆差异表达基因已成为现代分子生物学研究的热点 ,亦是功能基因组学研究的重要内容之一。抑制消减杂交技术以其低假阳性率、高灵敏度等优点 ,被广泛应用于生物及医学领域中差异表达基因的克隆 Gene expression changes are the core mechanisms regulating cell life activities. Isolating and cloning differentially expressed genes has become a hot research topic in molecular biology and one of the important aspects of functional genomics research. Suppression subtractive hybridization is widely used in the cloning of differentially expressed genes in biology and medicine due to its low false positive rate and high sensitivity