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植物水解蛋白是一种典型的蛋白质含氮掺假物质,被不法商贩作为廉价的蛋白质添加到牛奶中以提高其蛋白质含量。本研究自行配置不同浓度的植物水解蛋白掺假牛奶,应用近红外光谱分析仪漫反射模块直接扫描掺假样品,同时使用透射分析模块扫描三氯乙酸前处理的掺假样品;应用TQ软件分别建模并比较两种方法差异。结果表明:使用一阶导数&Norris Derivative滤波预处理,在8000~5000 cm~(-1),主成分数10,使用PCR构建了最优植物水解蛋白漫反射定量分析模型,RMSEC、RMSEP和R分别为0.224、0.214和0.98414,平均回收率为93.2479%。使用一阶导数&Norris Derivative滤波预处理,在10000~7000 cm~(-1),主成分数10,使用PCR构建了最优植物水解蛋白透射定量分析模型,RMSEC、RMSEP和R分别为0.175、0.138和0.99036,平均回收率为98.7351%。经过三氯乙酸处理的透射模型更加精确和稳定,可以用于牛奶中植物水解蛋白的检测,可以为牛奶品质控制和快速定量提供参考。
Phyto-protein hydrolysates, a typical nitrogen-containing adulterated substance, are added to milk by unscrupulous traders as an inexpensive protein to increase their protein content. In this study, different concentrations of plant protein hydrolyzate adulterated milk were configurated by ourselves. Adulterated milk samples were directly scanned by diffuse reflectance module of near-infrared spectrometer. At the same time, the adulterated samples of trichloroacetic acid pretreatment were analyzed by transmission analysis module. And compare the two methods of difference. The results showed that the optimal model of plant protein hydrolysable diffuse reflectance was established by PCR using the first derivative and Norris Derivative pretreatment. The RMSEC, RMSEP and R were respectively constructed by PCR at 8000-5000 cm -1 and the main composition of 10 0.224,0.214 and 0.98414, the average recovery was 93.2479%. Using the first derivative and Norris Derivative filter pretreatment, the optimal phytohemagglutinin transmission quantitative analysis model was constructed by PCR at 10000-7000 cm -1 and the main component was 10. The RMSEC, RMSEP and R were 0.175,0.138 And 0.99036, the average recovery was 98.7351%. The trichloroacetic acid transmission model is more accurate and stable, which can be used for the detection of plant protein hydrolysates in milk. It can provide a reference for quality control and rapid quantification of milk.