目的:对人参不定根的摇瓶培养条件进行系统的优化。方法:利用组织培养技术结合高效液相色谱法和紫外分光光度法,考察了接种量、蔗糖浓度以及无机盐浓度对人参不定根的生长、人参皂苷以及人参多糖合成的影响。结果:每1 L培养基接种的不定根鲜重为20 g时人参不定根的干重增殖倍数达到最大值;随着蔗糖浓度的升高,人参不定根干重增殖倍数呈先升高后降低的趋势,人参多糖含量增长趋势不明显,不同蔗糖浓度时各单体皂苷含量有显著区别,人参总皂苷含量随蔗糖浓度的升高而降低,单位体积培养基中的多糖和皂苷产量均在40 g.L-1蔗糖质量浓度下达到最大值;培养基中的无机盐浓度对不定根的生长以及多糖和皂苷的合成与积累都有较大影响,3/4MS最有利于不定根的生长以及皂苷的合成,而不定根中的多糖含量则随着盐浓度的升高而降低。结论:接种量、蔗糖浓度、无机盐浓度都会显著影响人参不定根的生长以及其活性成分的合成和积累。 OBJECTIVE: To systematically optimize culture conditions of flask-growing flask with adventitious roots. Methods: The effects of inoculum size, sucrose concentration and inorganic salt concentration on the adventitious root growth, ginsenosides and ginseng polysaccharides synthesis were studied by tissue culture technique combined with high performance liquid chromatography and ultraviolet spectrophotometry. Results: When the fresh weight of adventitious roots inoculated per 1 L of medium was 20 g, the multiplication of dry weight of adventitious roots reached the maximum. With the increase of sucrose concentration, the multiplication of dry weight of adventitious roots increased firstly and then decreased, Ginseng polysaccharides content growth trend is not obvious, different sucrose concentrations of each monomer saponin content were significantly different, ginseng total saponin content with increasing sucrose concentration decreased per unit volume of the medium polysaccharide and saponin production were 40 gL-1 the maximum concentration of sucrose; inorganic salts have a greater impact on the concentration in the medium and the growth of adventitious roots synthesis and accumulation of polysaccharide and saponin, 3 / 4MS most favorable to the growth of adventitious roots as well as synthetic saponin, and adventitious roots The polysaccharide content decreased with the increase of salt concentration. CONCLUSION: Inoculum size, sucrose concentration and inorganic salt concentration all significantly affect the growth of adventitious roots and the synthesis and accumulation of its active ingredients.