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目的 研究亚硒酸钠对氯化镉诱导的离体大鼠肝细胞DNA损伤的影响。方法 用8 75 μmol/L、17 5 0 μmol/L和 35 0 0 μmol/L的亚硒酸钠分别与 8 75 μmol/L、17 5 0 μmol/L和 35 0 0μmol/L的氯化镉联合作用 ,用单细胞凝胶电泳检测大鼠肝细胞DNA损伤。 结果 8 75 μmol/L的亚硒酸钠对 8 75 μmol/L、17 5 0 μmol/L和 35 0 0 μmol/L的氯化镉引起的DNA损伤都有拮抗作用 ;17 5 0 μmol/L的亚硒酸钠对 17 5 0 μmol/L和 35 0 0 μmol/L的氯化镉显示拮抗作用 ,而对 8 75μmol/L氯化镉拮抗作用不明显 ;当亚硒酸钠为 35 0 0 μmol/L时 ,则对 8 75 μmol/L、17 5 0 μmol/L和35 0 0 μmol/L的氯化镉没有明显拮抗作用。从氯化镉的角度 ,当氯化镉为 8 75 μmol/L时 ,只有8 75 μmol/L亚硒酸钠拮抗作用最明显 ;当氯化镉为 17 5 0 μmol/L和 35 0 0 μmol/L时 ,8 75 μmol/L和 17 5 0 μmol/L的亚硒酸钠有拮抗作用 ,17 5 0 μmol/L的亚硒酸钠的拮抗作用又优于 8 75 μmol/L的亚硒酸钠。结论 一定剂量的亚硒酸钠拮抗一定剂量的氯化镉引起的DNA损伤与亚硒酸钠的剂量有关 ,随剂量增加 ,拮抗作用下降 ,而且也与亚硒酸钠和氯化镉的相对剂量有关。
Objective To investigate the effect of sodium selenite on DNA damage induced by cadmium chloride (CdCl2) in isolated rat hepatocytes. Methods The effects of 8 75 μmol / L, 17 5 0 μmol / L and 35 0 0 μmol / L sodium selenite and 8 75 μmol / L, 175 μmol / L and 35 000 μmol / L cadmium chloride In combination, single-cell gel electrophoresis was used to detect DNA damage in rat hepatocytes. Results Selenite at 8 75 μmol / L had antagonistic effect on DNA damage induced by 8 75 μmol / L, 17 5 0 μmol / L and 35 0 0 μmol / L cadmium chloride. 17 5 0 μmol / L Of sodium selenite showed antagonism to cadmium chloride at 1750 μmol / L and 35000 μmol / L, but did not show obvious antagonism to 875 μmol / L cadmium chloride. When sodium selenite was 3500 μmol / L, there was no obvious antagonism to 8 75 μmol / L, 17 5 0 μmol / L and 35 000 μmol / L cadmium chloride. From the viewpoint of cadmium chloride, only 8 75 μmol / L sodium selenite showed the most obvious antagonism when cadmium chloride was 8 75 μmol / L; when cadmium chloride was 17 50 μmol / L and 35 000 μmol / L, selenite at 8 75 μmol / L and 17 5 0 μmol / L had antagonistic effects, and antagonism of selenite at 17 0 0 μmol / L was superior to selenium at 8 75 μmol / L Sodium acid. Conclusion A certain dose of sodium selenite antagonizes the DNA damage caused by a certain dose of cadmium chloride, which is related to the dose of sodium selenite. With the increase of dose, the antagonism decreases, but also with the relative dose of sodium selenite and cadmium chloride related.