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本文用正交试验法,建立了由番泻叶中浸出总番泻苷的醇回流提取方法。优选出最佳提取条件:乙醇浓度为55%,药物与醇的比例为1:12.5(g:ml),回流时间为第一次15min,第二次10min,浸泡时间为omin。提取效率考察证明:该法提取总番泻苷为其含量的99.64%。
In this paper, orthogonal experiments were used to establish an alcohol reflux extraction method for leaching total sennoside from senna leaf. The optimum extraction conditions were as follows: ethanol concentration 55%, drug to alcohol ratio 1:12.5 (g:ml), reflux time for the first 15 min, second time 10 min, and soaking time omin. The extraction efficiency inspection proves that this method extracts 99.64% of the total sennoside content.