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目的:建立测定双黄祛毒片(盐酸小檗碱、大黄、五倍子)含量的方法。方法:采用HPLC在同一色谱条件ZORBAX SB-Pheny色谱柱(4.6 mm×150 mm,5μm)对没食子酸、盐酸小檗碱、大黄酸、大黄酚、芦荟大黄素、大黄素、大黄素甲醚进行含量测定。结果:线性范围分别为0.12～2.4μg(r=0.999 9),盐酸小檗碱线性范围0.11～2.2μg(r=0.999 9),0.015～0.3μg(r=0.999 8),0.02～0.4μg(r=0.999 8),0.008～0.16μg(r=0.999 9),0.013～0.26μg(r=0.999 8),0.006～0.12μg(r=0.999 9),平均回收率分别为100.97%,100.20%,99.01%,99.76%,101.10%,101.97%,101.56%。结论:该方法简便、快速、准确,重复性好,可作为双黄祛毒片中没食子酸、盐酸小檗碱、大黄酸、大黄酚、芦荟大黄素、大黄素、大黄素甲醚含量测定方法。
Objective: To establish a method for determination of Shuanghuadudu tablets (berberine hydrochloride, rhubarb, Chinese nutgall) content. Methods: Gallic acid, berberine, rhein, chrysophanol, aloe emodin, emodin and physcion were separated by HPLC on a ZORBAX SB-Pheny column (4.6 mm × 150 mm, 5 μm) Determination of content. RESULTS: The linear range was 0.12-2.4μg (r = 0.999 9), the linear range of berberine hydrochloride was 0.11-2.2μg (r = 0.999 9), 0.015-0.3μg (r = 0.999 8), 0.02-0.4μg (r = 0.999 8), 0.008-0.16μg (r = 0.999 9), 0.013-0.26μg (r = 0.999 8) and 0.006-0.12μg (r = 0.999 9) .The average recoveries were 100.97%, 100.20% 99.01%, 99.76%, 101.10%, 101.97%, 101.56%. Conclusion: The method is simple, rapid, accurate and reproducible. It can be used as a method for the determination of gallic acid, berberine hydrochloride, rhein, chrysophanol, aloe emodin, emodin and physcion in Shuanghuadudu Tablet .