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目的研究酪氨酸激酶Etk/BMX转染对鼻咽癌细胞增殖的影响。方法利用脂质体2000将野生型酪氨酸激酶Etk/BMX表达质粒pcDNA3.1-Etk导入Etk/BMX低表达水平的鼻咽癌细胞株sune-1中,建立Etk/BMX稳定高表达细胞株sune-wt,空质粒pcDNA3.1转染细胞作为对照(sune-vector)。流式细胞仪检测其转染效率,然后分别进行克隆平板形成率试验和裸鼠成瘤试验。结果成功筛选到持续高表达Etk的sune-wt细胞,流式细胞仪检测Etk,表达率为(75.5±2.34)%。克隆平板形成率试验sune-wt较之sune-1增殖率较高,裸鼠成瘤试验,sune-wt较之sune-vector和sune-1肿瘤生长较快,sune-vector和sune-1肿瘤生长差异无统计学意义。结论 Etk/BMX在鼻咽癌中可促进细胞增殖。
Objective To investigate the effect of tyrosine kinase Etk / BMX on the proliferation of nasopharyngeal carcinoma cells. Methods The wild-type tyrosine kinase Etk / BMX expression plasmid pcDNA3.1-Etk was transfected into the nasal carcinoma cell line sune-1 with low expression level of Etk / BMX by lipofectamine 2000. The stable highly expressed Etk / BMX cell line sune-wt, empty plasmid pcDNA3.1 transfected cells as a control (sune-vector). The transfection efficiency was detected by flow cytometry, and then the rate of clone plate formation and tumor formation in nude mice were respectively tested. Results The sune-wt cells with high expression of Etk were successfully screened, and the expression of Etk was detected by flow cytometry (75.5 ± 2.34)%. Compared with sune-1, the growth rate of sune-wt was higher than that of sune-1 in nude mice. Sune-wt grew faster than sune-vector and sune-1 and sune-vector and sune- The difference was not statistically significant. Conclusion Etk / BMX can promote cell proliferation in nasopharyngeal carcinoma.