本文报告了一个从携带者血清中大量提取HBsAg的方法。首先,用5.5%聚乙二醇6000使Dane颗粒和主要的脂蛋白沉淀;第二步用11%聚乙二醇把HBsAg沉淀出来;第三步用溴化钾作漂浮密度梯度区带离心,和第四步用蔗糖密度梯度速率区带纯化所得的HBsAg光密度单位剩余为0.32%,也就是去除了99.68%杂蛋白,回收率为73.4%。 This article reports a method for mass extraction of HBsAg from the serum of carriers. First, Dane granules and major lipoproteins are precipitated with 5.5% polyethylene glycol 6000; secondly, HBsAg is precipitated with 11% polyethylene glycol; thirdly, centrifugation is carried out using potassium bromide as a floating density gradient zone, And in the fourth step, the remaining HBsAg densities in the zone of sucrose density gradient were 0.32%, that is, 99.68% of the hybrid proteins were removed with a recovery of 73.4%.