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目的探讨细粒棘球绦虫(Eg)重组双歧杆菌(Bb)-Eg95-EgA31疫苗免疫和Eg原头节攻击后小鼠的囊重抑制率及脾细胞增殖的变化。方法将细粒棘球绦虫重组Bb-Eg95-EgA31疫苗分别采用皮下注射、肌肉注射、鼻腔内接种和口服灌胃4种途径免疫BALB/c小鼠,免疫后8周每鼠用50个Eg原头节攻击感染,感染后25周剖杀小鼠,分离细粒棘球蚴包囊并称重,计算囊重抑制率;取脾,分离脾细胞,用Eg粗抗原(EgAg)或刀豆素A(ConA)刺激培养,四甲基偶氮唑盐比色法(MTT法)检测免疫小鼠脾细胞增殖反应,同时设有空载体、Bb和MRS对照。结果以上4种疫苗接种组小鼠的囊重抑制率分别为45.33%、41.33%、70.67%和62.67%;疫苗接种组的脾细胞明显增殖;鼻腔内接种和口服免疫组的脾细胞增殖显著高于皮下和肌肉注射组。结论细粒棘球绦虫重组Bb-Eg95-EgA31疫苗可诱导小鼠产生特异性的细胞免疫反应。
Objective To investigate the inhibitory rate of cystic echinococcosis and the proliferation of splenocytes in mice immunized with recombinant Bifidobacterium Egghi (Egb) -Eg95-EgA31 and Eg protoscoiitis. Methods BALB / c mice were immunized with recombinant Bb-Eg95-EgA31 vaccine by subcutaneous injection, intramuscular injection, intranasal inoculation and oral gavage respectively. After immunization for 8 weeks, 50 Eggen The heads were challenged and challenged. The mice were killed 25 weeks after infection. Echinococcus granulosus was isolated and weighed, and the inhibition of cystic weight was calculated. The spleens were isolated and spleen cells were isolated. EgAg or concanavalin A (ConA) -stimulated culture. The proliferation of splenocytes in immunized mice was detected by MTT colorimetric assay. Meanwhile, empty vector, Bb and MRS control were also prepared. Results The inhibition rates of the capsule weight of the four vaccinated groups were 45.33%, 41.33%, 70.67% and 62.67%, respectively. The spleen cells of the vaccinated group were significantly proliferated. The proliferation of spleen cells in the intranasal vaccination and oral immunization groups was significantly higher Subcutaneous and intramuscular injection groups. Conclusion Echinococcus granulosus recombinant Bb-Eg95-EgA31 vaccine can induce specific cellular immune response in mice.