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探讨北京与河南地区丙型肝炎病毒(HCV)基因型分布情况以及HCV 5’-NCR和HCV NS5b两区域分型结果之间的差异。对56例慢性丙型肝炎和80例有偿供血员的HCV RNA阳性血清。同时进行HCV 5’-NCR和HCV NS5b酶切分型研究。HCV 5’-NCR和HCV NS5b两区域的Ⅱ/1b型感染率北京地区分别占85.7％和87.5％,河南地区分别占68.8％和70.0％。HCV Ⅱ/lb型为北京和河南地区HCV感染的优势株,同时HCV 5’-NCR和HCV NS5b区酶切分型结果间具有良好的相互对应关系。
To investigate the distribution of hepatitis C virus (HCV) genotypes in Beijing and Henan Province and the differences between the two regions of HCV 5’-NCR and HCV NS5b. HCV RNA positive sera of 56 chronic hepatitis C and 80 paid blood donors were collected. At the same time, HCV 5’-NCR and HCV NS5b digestion typing studies. The prevalence of type II / 1b infection in both HCV 5’-NCR and HCV NS5b regions was 85.7% and 87.5% in Beijing and 68.8% and 70.0% respectively in Henan. The HCV Ⅱ / 1b type was the predominant strain of HCV infection in Beijing and Henan Provinces, and the reciprocal correspondence between the results of restriction enzyme digestion and sequencing of HCV 5’-NCR and HCV NS5b.