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目的分析华支睾吸虫感染新西兰兔转化生长因子β1(TGF-β1)、白细胞介素17(IL-17)的变化及意义。方法选择健康新西兰兔8只,经口灌胃华支睾吸虫囊蚴800个;于感染0、1、3、7、14、21、28和42d耳缘静脉采血,分离血清,用ELISA法检测血清中TGF-β1、IL-17水平;于42d剖杀感染兔,取肝脏制作组织切片,HE染色观察病理变化。结果实验兔感染0d时TGF-β1为(706.08±60.72)pg/ml,感染42d时为(1106.26±60.35)pg/ml,差异有统计学意义(P<0.01)。IL-17水平自感染开始持续增高,在感染3d时[(68.69±6.04)pg/ml]达到最高峰,与感染0d[(50.04±4.16)pg/ml]时相比,差异有统计学意义(P<0.05);此后逐渐降低,至21d时降至感染前水平。结论新西兰兔感染华支睾吸虫早期IL-17高表达,感染42d时TGF-β1高表达。提示TGF-β1/IL-17在华支睾吸虫致病过程中发挥重要作用。
Objective To analyze the changes of TGF-β1 and IL-17 in New Zealand rabbits infected with Clonorchis sinensis. Methods Eight healthy New Zealand rabbits were selected and orally administered intraperitoneally with 800 metacercaria of Clonorchis sinensis. Serum was collected from the ventral marginal veins at 0, 1, 3, 7, 14, 21, 28 and 42 days after infection. Serum was separated and detected by ELISA Serum levels of TGF-β1, IL-17; infected infected rabbits at 42d, the liver tissue sections were made, HE staining to observe the pathological changes. Results The level of TGF-β1 was (706.08 ± 60.72) pg / ml on day 0 of infection in rabbits and (1106.26 ± 60.35) pg / ml on the 42th day of infection. The difference was statistically significant (P <0.01). The level of IL-17 began to increase from the beginning of infection, reaching the peak at [(68.69 ± 6.04) pg / ml] on 3d, which was significantly different from that at 0d [(50.04 ± 4.16) pg / (P <0.05), then decreased gradually, and then decreased to pre-infection level by 21 days. Conclusion New Zealand rabbit infected with Clonorchis sinensis early high expression of IL-17, 42d infected with high expression of TGF-β1. These results suggest that TGF-β1 / IL-17 plays an important role in the pathogenesis of Clonorchis sinensis.