外分泌液中SIgA的分离与定量测定一直是基础与临床研究和应用中的主要困难。我们用蔗糖密度梯度超速离心和RIA相结合的方法对唾液SIgA进行了分离和定量测定,结果表明本法能有效分离SIgA与IgA,具有较好的稳定性、准确性和可重复性,可同时获得唾液SIgA、IgA、总IgA含量与SIgA、IgA各占总IgA含量的百分比五项指标,较全面反映唾液SIgA的改变情况。本文方法为唾液SIgA的研究与应用提供了有效的技术手段。 Separation and quantitation of SIgA in exocrine fluid has been a major difficulty in basic and clinical research and applications. We isolated and quantified salivary SIgA by sucrose density gradient ultracentrifugation and RIA. The results showed that this method can effectively separate SIgA and IgA, and has good stability, accuracy and repeatability, Saliva SIgA, IgA, total IgA content and SIgA, IgA each of the total IgA content of the five indicators, more fully reflect the saliva SIgA changes. This method provides an effective technical means for the research and application of salivary SIgA.