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目的:建立RP-HPLC同时测定香青兰提取物中4种黄酮成分含量的方法。方法:Sunfire-C18色谱柱(250 mm×4.6mm,5μm);以乙腈(A)-0.2%磷酸(B)水溶液为流动相,线性梯度洗脱(0~5 min,16%A;5~45 min,16%→28%A;45~65 min,28%→80%A);流速1.05 mL·min-1;柱温35℃;检测波长330 nm。结果:香叶木素-7-O-β-D-葡萄糖醛酸苷、田蓟苷、刺槐素-7-O-β-D-葡萄糖醛酸苷和刺槐素质量浓度分别在1.20~24.00μg·mL-1(r=0.999 8),9.03~180.48μg·mL-1(r=0.999 9),1.00~20.00μg·mL-1(r=0.999 9)和0.83~16.64μg·mL-1(r=0.999 9)范围内呈良好的线性关系;平均回收率分别为99.62%,100.11%,98.86%,102.51%,RSD分别为1.10%,1.50%,0.88%,1.72%。结论:该方法简单、准确可靠,可为香青兰提取物中黄酮成分的含量测定提供科学依据。
Objective: To establish a method for the simultaneous determination of four flavonoids in the extract of Ching Ching Lam by RP-HPLC. Methods: A linear gradient elution was performed on a Sunfire-C18 column (250 mm × 4.6 mm, 5 μm) using a linear gradient of acetonitrile-A0.2% phosphoric acid (B) 45 min, 16% → 28% A; 45-65 min, 28% → 80% A); the flow rate was 1.05 mL · min-1; Results: The concentrations of geraniol -7-O-β-D-glucuronide, thymidine, acaciacin-7-O-β-D-glucuronide and acacia were 1.20-24.00 μg · mL (r = 0.999 8), 9.03-180.48 μg · mL -1 (r = 0.999 9), 1.00-20.00 μg · mL -1 (r = 0.999 9) and 0.83-16.64 μg · mL -1 = 0.999 9). The average recoveries were 99.62%, 100.11%, 98.86% and 102.51%, respectively. The RSDs were 1.10%, 1.50%, 0.88% and 1.72%, respectively. Conclusion: The method is simple, accurate and reliable, which can provide a scientific basis for the determination of flavonoids content in the extract.