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目的：研究司来吉兰（ｓｅｌｅｇｉｌｉｎｅ，Ｓｅｌ）的神经保护作用及其对单胺氧化酶Ｂ（ＭＡＯＢ）的抑制作用．方法：高效液相电化学检测法测定小鼠纹状体内多巴胺（ＤＡ）及其代谢产物的含量．荧光检测法测定小鼠脑ＭＡＯＢ的活性．结果：ＭＰＴＰ（３０ｍｇ·ｋｇ－１ｉｐ）明显减少小鼠纹状体内ＤＡ含量（７３％）．预先给予Ｓｅｌ（１０ｍｇ·ｋｇ－１ｉｐ）可以阻断ＭＰＴＰ的神经毒性；而ＭＰＴＰ损伤后再给予Ｓｅｌ则否．ＭＰＴＰ损伤后２ｗｋ内，Ｓｅｌ对纹状体ＤＡ水平的自然恢复无促进作用．ＭＰＰ＋外周给药无神经毒性．Ｓｅｌ对小鼠脑内ＭＡＯＢ有明显抑制作用（ＩＣ５０＝３８；９５％可信限为：３１－４５μｇ·Ｌ－１）．结论：Ｓｅｌ具有与其抑制脑内ＭＡＯＢ活性相关的对抗ＭＰＴＰ毒性的神经保护作用．
Objective: To study the neuroprotective effect of selegiline (Sel) and its inhibitory effect on monoamine oxidase B (MAOB). Methods: The contents of dopamine (DA) and its metabolites in mouse striatum were determined by high performance liquid phase electrochemical detection. Fluorescence detection of mouse brain MAO B activity. Results: MPTP (30 mg · kg-1 ip) significantly reduced the content of DA in the striatum of mice (73%). Pretreatment with Sel (10 mg · kg-1 ip) blocked the neurotoxicity of MPTP; whereas Sel did not give MPTP after injury. Within 2 weeks after MPTP injury, Sel had no effect on the natural recovery of striatal DA levels. MPP + peripheral administration without neurotoxicity. Sel on mouse brain MAO B significantly inhibited (IC50 = 3 8; 95% confidence limits: 3 1 -4 5μg · L-1). Conclusion: Sel has neuroprotective effects against MPTP toxicity associated with its inhibition of brain MAO-B activity.