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通过水培实验研究了钒(V)、汞(Hg)单一和复合胁迫对四九黄菜心幼苗生理生化特性的影响。结果表明:随着胁迫强度的增加,丙二醛(MDA)含量和细胞膜透性明显上升,与对照相比,MDA含量和细胞膜透性分别增加了8.4%～271.5%和22.4%～145.6%;叶绿素含量、过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶(SOD)活性均呈先上升后下降的趋势;重金属浓度较低时,3种保护酶均有较好的协同效应,表现出较强的自我调节能力;重金属浓度较高时,叶绿素含量和3种酶的活性呈下降趋势,表明在逆境环境因子下,植物的生理特征发生变化,酶活性受到抑制;V、Hg复合胁迫对蔬菜幼苗的毒害作用大于同水平单一胁迫效应。
The effects of single and combined stresses of vanadium (V) and mercury (Hg) on the physiological and biochemical characteristics of Brassica campestris L. seedlings were studied by hydroponic experiments. The results showed that MDA content and membrane permeability increased significantly with the increase of stress intensity. Compared with the control, MDA content and membrane permeability increased by 8.4% -271.5% and 22.4% -145.6%, respectively. The activities of chlorophyll, CAT, POD and SOD all increased first and then decreased. When the concentrations of heavy metals were low, the activities of three protective enzymes Good synergistic effect, showing a strong ability of self-regulation; higher concentrations of heavy metals, chlorophyll content and activity of three enzymes showed a downward trend, indicating that under adverse environmental factors, the physiological characteristics of plants change, inhibition of enzyme activity ; V, Hg composite stress on vegetable seedlings poisoning effect is greater than the same level of single stress effect.