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目的研究循环Ghrelin及胃黏膜Proghrelin表达与幽门螺杆菌(Hp)感染及胃溃疡、胃腺癌等Hp感染相关疾病之间的关系。方法使用放射免疫分析及RT-PCR测定10例Hp感染阴性健康志愿者及35例Hp感染阳性患者(其中胃溃疡11例,胃癌13例,无明显病灶者11例)的血浆Ghrelin水平及胃黏膜Proghrelin mRNA的表达。结果在本研究中,各种Hp表达阳性的病例中循环Ghrelin水平及胃黏膜Proghrelin mRNA的表达要低于Hp阴性的健康志愿者(P<0.05),与其他Hp感染患者相比,胃癌患者循环Ghrelin水平更低(P<0.05),而胃黏膜Proghrelin mRNA的表达却无明显差异。结论Hp能够降低胃黏膜Proghrelin的表达以及循环Ghrelin水平。
Objective To study the relationship between the expression of circulating Ghrelin and gastric mucosa Proghrelin and Helicobacter pylori (Hp) infection and diseases related to Hp infection such as gastric ulcer and gastric adenocarcinoma. Methods The radioimmunoassay and RT-PCR were used to detect the plasma levels of Ghrelin and gastric mucosa in 10 Hp-negative healthy volunteers and 35 Hp-positive patients (11 gastric ulcer, 13 gastric cancer and 11 non-obvious gastric cancer) Proghrelin mRNA expression. Results In this study, the circulating Ghrelin levels and the expression of Proghrelin mRNA in Hp-positive cases were lower than those in Hp-negative healthy volunteers (P <0.05). Compared with other Hp-infected patients, the gastric cancer patients were cycled Ghrelin level was lower (P <0.05), while the expression of Proghrelin mRNA in gastric mucosa had no significant difference. Conclusion Hp can reduce the expression of Proghrelin and the level of circulating Ghrelin in gastric mucosa.