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目的:研究不同剂量不同时间铁、铝、铅元素神经细胞生存能力的影响和Vit C在铁、铝、铅元素诱导神经细胞凋亡中的作用。为探讨微量元素生理功能提供理论依据和资料。方法:采用体外培养脑胶质瘤细胞,MTT比色法测定40μmol/L、400μmol/L浓度下的三氯化铝、硫酸亚铁、醋酸铅3种药物及3种药物联合250μmol/L Vit C对脑胶质瘤细胞体外生长的影响。结果:24 h醋酸铅、三氯化铝联合维生素C实验组,细胞生长抑制作用明显大于400μmol/L和40μmol/L浓度组。最弱为40μmol/L浓度硫酸亚铁,细胞数量明显高于联合Vit C和400μmol/L浓度组。72 h各实验组对细胞生长抑制作用减弱,40μmol/L浓度的硫酸亚铁细胞生长数量明显高于其他实验组。除硫酸亚铁+Vit C、40μmol/L浓度组与对照组无统计学意义,其他各实验组细胞数量明显低于对照组。结论:铝离子、高浓度铅离子和高浓度铁对肿瘤细胞的生长有明显的抑制作用,Vit C能增强铁、铝、铅离子诱导胶质细胞凋亡。其中Vit C对铝离子的效应大于铅离子和铁离子。
OBJECTIVE: To investigate the effects of different doses of iron, aluminum and lead on the viability of neurons and on the apoptosis of neurons induced by iron, aluminum and lead. In order to explore the physiological functions of trace elements to provide a theoretical basis and information. Methods: Glioma cells were cultured in vitro. MTT colorimetry was used to determine the concentrations of aluminum trichloride, ferrous sulfate and lead acetate at 40μmol / L and 400μmol / L, and three drugs combined with 250μmol / L Vit C Effect of in vitro growth of glioma cells. Results: 24 h lead acetate, aluminum trichloride combined with vitamin C experimental group, the cell growth inhibitory effect was significantly greater than 400μmol / L and 40μmol / L concentration group. The weakest was 40μmol / L ferrous sulfate, the cell number was significantly higher than the combination of Vit C and 400μmol / L concentration group. At 72 h, the growth inhibition of cells in each experimental group was weakened, and the number of ferrous sulfate cells grown in the concentration of 40 μmol / L was obviously higher than that of other experimental groups. In addition to the ferrous sulfate + Vit C, 40μmol / L concentration group and the control group was not statistically significant, the other experimental groups of cells was significantly lower than the control group. CONCLUSION: Aluminum ions, high concentration of lead ions and high concentration of iron have obvious inhibitory effect on the growth of tumor cells. Vit C can enhance the apoptosis of glial cells induced by iron, aluminum and lead ions. The effect of Vit C on aluminum ions is greater than that of lead ions and iron ions.