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目的 将活血通腑方药剂用于腹腔粘连大鼠 ,通过血浆纤维蛋白原 (FIB)含量、TGF βmRNA表达的检测和大鼠腹腔粘连级别评分 ,确立活血通腑方有效剂量。方法 将模型SD(清洁级 )雄性大鼠随机分为大、中、小剂量 3组 ,进行胃内药物灌注 ,观察d 7大鼠腹腔粘连情况 ,测定血浆FIB含量、粘连组织标本TGF βmRNA表达 ,筛选出活血通腑方的有效剂量。结果 ①血浆FIB的析出中、小、大剂量组呈递增态势 ;②粘连级别中、小、大剂量组顺序递增 ;③TGF βmRNA表达大、中、小剂量组递减。结论 中剂量活血通腑方能影响大鼠术后腹腔粘连程度、降低血浆FIB含量及TGF βmRNA表达。
Objective To use Huoxue Tongbi prescription for intraperitoneal adhesion rats, and to establish the effective dose of Huoxue Tongyu prescription by measuring the content of fibrinogen (FIB), the expression of TGF β mRNA, and the rat peritoneal adhesion grade score. Methods SD SD rats were randomly divided into three groups: large, medium, and low doses. Gastric perfusion was performed to observe intraperitoneal adhesions in d 7 rats. FIB content in plasma and TGF β mRNA expression in adherent tissue samples were measured. The effective dose of Huoxue Tongyu Fang was selected. Results 1The levels of plasma FIB in the middle, small and high dose groups increased gradually; 2 medium-, small-, and high-dose groups increased gradually; 3 TGF-β mRNA decreased in large, medium and small dose groups. Conclusion The middle-dose Huoxuetongyu recipe can affect the degree of intra-abdominal adhesion, decrease the content of FIB in plasma and the expression of TGF-βmRNA in rats.