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评价冬小麦抗冻性鉴定的方法包括田间直接鉴定,人工气候模拟鉴定和间接鉴定方法。田间成活是越冬作物杭寒性测定最基本的方法,人工冷冻技术可以减少基因型与环境因素的相互作用,利用冷冻成活率可以作为评价遗传型抗冻性的标准,评价了冬小麦15个生理、生化和形态性状,证明许多性状与田间成活指数存在密切的线性关系。说明被筛选出来的这些性状。能够对田间成活测定的可靠性加以补充说明。 本研究中。发现半致死溫度(LT_(50)),分蘖节含水量(CH_2O),叶片含水量(LH_2O),直立性(ERECT),分孽节入土深度(CDEP),电导率(EC)钾离子含量(K~+),细胞液浓度(CSC)和存活率(SR)与田间成活指数高度相关,相关系数分別为-0.7963,-0.7986,-0.8217,-0.8512,-0.8924,-0.6552,-0.6681,0.8650和0.8415。 本文对电解质渗出率曲线配以logistic方程,并利用求拐点溫度来计算LT_(50),与田间直接鉴定结果基本吻合,组织含水量,幼苗匍匐性,K~+含量,测定结果不仅和田间成活指数接近而且快速简单,重演性好,适于大批量品种鉴定。
Methods to evaluate the winter wheat frost resistance identification include direct field identification, artificial climate simulation and indirect identification methods. Field survival is the most basic method for determining the coldness of winter crops. Artificial freezing technique can reduce the interaction between genotypes and environmental factors. Using the frozen survival rate as a standard to evaluate the genetic type of frost resistance, 15 winter wheat physiological, Biochemical and morphological traits, to prove that many traits and field survival index there is a close linear relationship. Explain these traits that have been screened out. The reliability of field-based assays can be complemented. In this study. (LT 50), water content (CH 2 O), leaf water content (LH 2 O), ERECT, CDEP, EC K + K ~ +), cell concentration (CSC) and survival rate (SR) were highly correlated with the field survival index, the correlation coefficients were -0.7963, -0.7986, -0.8217, -0.8512, -0.8924, -0.6552, -0.6681 and 0.8650 And 0.8415. In this paper, the electrolyte exudation rate curve was fitted with logistic equation, and the temperature of inflection point was used to calculate LT_ (50), which was in good agreement with the direct identification results of the field, the tissue water content, the seedling creep and K ~ + content. Survival index close to and quick and easy, good repeatability, suitable for large quantities of species identification.