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目的 瘤基因 (WT1)突变与白血病发病的关系。方法 采用PCR -SSCP技术检测 32例白血病病人标本 ,其中小于 16岁的患者 9例 ,成人 2 3例 (平均年龄 33岁 ) ;急性淋巴细胞白血病 15例 ,慢性粒细胞性白血病 8例 ;18例正常人血标本。结果 有 3例白血病标本的PCR产物即WT1基因电泳迁移异常 :1例为急性淋巴细胞白血病患者、2例为急性粒细胞性白血病患者 ;分别位于外显子 7、6和 10。临床追踪观察发现该 3例白血病患者经化疗未能达到完全缓解 ,全部于发病后 3个月内死亡 ,而WT1基因正常的白血病患者经化疗后大多数能达到部分缓解或完全缓解 ,生存期明显延长。正常血标本PCR产物和SSCP结果无明显异常。结论 WT1基因突变与白血病发病有关 ,有助于白血病辅助诊断和预后的分析。
The relationship between the gene mutation of WT1 and the leukemia. Methods PCR-SSCP was used to detect 32 leukemia patients, including 9 patients younger than 16 years old, 23 adults (mean age 33 years), 15 acute lymphoblastic leukemia, 8 chronic myelogenous leukemia and 18 Normal blood samples. Results The PCR products of 3 leukemic samples, namely the WT1 gene, were abnormally migrated by electrophoresis. One patient had acute lymphoblastic leukemia and two patients had acute myeloid leukemia, located in exons 7, 6 and 10, respectively. Clinical follow-up found that the 3 cases of leukemia patients failed to achieve complete remission by chemotherapy, all within 3 months after the onset of death, and WT1 gene in normal patients with leukemia after chemotherapy can be partially or completely relieved, the survival was significantly extend. Normal blood samples PCR products and SSCP results no significant abnormalities. Conclusion The mutation of WT1 gene is associated with the pathogenesis of leukemia, which is helpful for the diagnosis and prognosis of leukemia.