论文部分内容阅读
目的探讨青霉胺和硫酸锌对正常人外周血淋巴细胞ＤＮＡ的损伤程度。②方法采用染色体分析技术，分别对对照组、青霉胺组（０．０１μｇ／Ｌ）、硫酸锌组（０．０２μｇ／Ｌ）和青霉胺＋硫酸锌组（青霉胺０．０１μｇ／Ｌ，硫酸锌０．０２μｇ／Ｌ）体外培养的淋巴细胞姐妹染色单体交换（ＳＣＥ）频率进行了观察。③结果对照组、青霉胺组、硫酸锌组和青霉胺＋硫酸锌组的ＳＣＥ计数平均值分别为５．４３±０．５３，８．１５±１．３６，５．６６±０．５９和６．７１±０．７４，除对照组与硫酸锌组之间差异无显著性（ｔ＝１．２６４，Ｐ＞０．０５）外，其他各组之间差异均有极显著性（ｔ＝４．０５４～８．１２０，Ｐ＜０．００１）。④结论硫酸锌对染色体稳定性无影响，青霉胺对ＤＮＡ损伤有一定的作用。
Objective To investigate the DNA damage of normal human peripheral blood lymphocytes by penicillamine and zinc sulfate. Methods Chromosomal analysis was used to detect the effect of penicillamine (0.01μg / L, 0.01μg / L), zinc sulfate (0.02μg / L) and penicillamine + zinc sulfate L, zinc sulfate 0.02μg / L) cultured in vitro lymphocytes sister chromatid exchange (SCE) frequency was observed. Results The average SCE counts of control group, penicillamine group, zinc sulfate group and penicillamine + zinc sulfate group were 5.43 ± 0.53, 8.15 ± 1.36 and 5.66 ± 0 respectively. 59 and 6.71 ± 0.74, except for the difference between the control group and the zinc sulfate group (t = 1.264, P> 0.05), the differences among the other groups were significant ( t = 4.054 ~ 8.120, P <0.001). ④ Conclusion Zinc sulfate has no effect on chromosome stability, penicillamine has a certain effect on DNA damage.