论文部分内容阅读
目的对不同温度下人体脾细胞DNA降解状态进行多参数联合定量分析,得出温度相关的多元回归方程。方法选取13例已知PMI的人体离体脾脏,在死后13h～34h内,分别在10℃、20℃及30℃条件下,每隔1h取材,进行细胞学涂片,Feulgen-Vans染色,选用图像分析系统检测几何参数(ID)及灰度参数(AOD、IOD、AG、LDC)指标,对不同温度的检测指标进行多元回归等统计分析,得出相应的回归方程。结果温度相关的脾细胞DNA降解推断死亡时间回归方程是:Y=-0.668619XT+2.31609XID-33.45671XAOD-1.13296XIOD+0.44433XAG(XT=10,20,30)。结论不同温度的脾细胞AOD、IOD、ID、AG均有显著性差异,温度及反映DNA含量的各个参数与PMI存在很强的相关性,可以将温度作为参数引入方程用于推断PMI。
Objective To quantitatively analyze the DNA degradation status of human spleen cells under different temperatures and get the temperature-dependent multiple regression equation. Methods Thirteen cases of PMI isolated from human spleen were selected and stained with Feulgen-Vans at 13h-34h after death at 10 ℃, 20 ℃ and 30 ℃, respectively. The image analysis system was used to detect geometric parameters (ID) and gray level parameters (AOD, IOD, AG, LDC) indicators, and multiple regression analysis of different temperature indicators to obtain the corresponding regression equation. Results The regression equation of temperature-dependent DNA degradation of splenocytes inferred death time was: Y = -0.668619XT + 2.31609XID -33.45671XAOD-1.13296XIOD + 0.44433XAG (XT = 10,20,30). Conclusion The AOD, IOD, ID and AG of splenocytes at different temperatures are significantly different. The parameters of temperature and DNA content are strongly correlated with PMI. The temperature can be introduced into the equation as a parameter to infer PMI.