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为研究细胞对药物的定性定量反应,并且能够形成浓度梯度,本研究利用软件CAD、软件CFD以及软件Fluent设计了一种新形式的浓度梯度微流控芯片,注入芯片的流体能够形成浓度梯度,该芯片添加了有细胞捕获结构。研究对芯片的影响因素进行了优化,用红绿染料对芯片的浓度梯度特征进行了验证,探讨带有细胞捕获结构的浓度梯度微流控芯片实现药物对内皮细胞凋亡的可行性。通过芯片平台研究了葡萄糖以及土豆叶匀浆液诱导内皮细胞凋亡的情况。研究结果表明,新设计的浓度梯度芯片经过红、绿染料的验证,在出口主通道有八通道溶液形成了层流特性,能够保持较长的距离,形成了明显的稳定的浓度梯度。在诱导内皮细胞凋亡方面,当葡萄糖的浓度大于25μmol/L时,细胞出现了明显的抑制;而8个浓度的土豆叶匀浆稀释液对诱导内皮细胞凋亡没有明显的作用。本研究设计的微流控芯片能够实现药物诱导细胞,为筛选肿瘤药物提供了新的途径。
In order to study the qualitative and quantitative response of cells to drugs and to establish a concentration gradient, a new type of concentration gradient microfluidic chip was designed by using software CAD, software CFD and software Fluent. The injected fluid could form a concentration gradient, The chip adds a cell-capture structure. The influence factors of the chip were optimized, and the concentration gradient of the chip was verified by using red and green dye. The feasibility of the drug on the apoptosis of endothelial cells was explored by concentration gradient microfluidic chip with cell capture structure. The apoptosis of endothelial cells induced by glucose and potato leaves homogenate was studied by chip platform. The results show that the new design of the concentration gradient chip through the red and green dye verification, the main channel in the exit of the eight-channel solution formed a laminar flow characteristics, to maintain a longer distance, the formation of a clear and stable concentration gradient. In the induction of endothelial cell apoptosis, when the glucose concentration is greater than 25μmol / L, the cells showed obvious inhibition; and 8 concentrations of potato leaf homogenate did not induce apoptosis of endothelial cells had no significant effect. The microfluidic chip designed in this study can induce drug-induced cells and provide a new way to screen oncology drugs.