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体内外实验证明,吡喹酮无明显的抑制成熟日本血吸虫卵孵化的作用,但可使毛蚴的活动能力受到影响或使其活动异常,为进一步探讨毛蚴生理特性的变化,我们观察了吡喹酮对毛蚴趋性的影响。 材料和方法 昆明种小白鼠(体重24～25g),每鼠感染日本血吸虫尾蚴40条。35d后随机取感染鼠3只,设为实验1、2、3组;每鼠从口腔灌注0.5ml 0.3％吡喹酮悬液(吡喹酮原粉溶于0.3％羧甲基纤维素钠中,药物浓度30mg/ml),实际灌注药量约600mg/kg。另取1只未经服药的小鼠,设为对照组。 投药24h后,解剖以上小鼠。参考Dresden法取其大肠各1g,并收集虫卵。取各鼠虫卵悬液2ml于小试管中孵化,30min后吸取等量管口毛蚴悬液投入实验。
In vitro and in vivo experiments showed that praziquantel did not significantly inhibit the egg hatching of mature Schistosoma japonicum but could affect the activity of miracidia or make it abnormal. To further explore the physiological characteristics of miracidia, we observed the effects of praziquantel Effect on the tendency of cercariae. Materials and methods Kunming mice (weight 24 ~ 25g), each infected with 40 of Schistosoma japonicum cercariae. After 35 days, 3 mice were randomly selected and infected. The mice were randomly divided into experimental groups 1, 2, and 3; 0.5ml of 0.3% praziquantel suspension was administered orally into the oral cavity (praziquantel powder was dissolved in 0.3% sodium carboxymethylcellulose , Drug concentration 30mg / ml), the actual perfusion dose of about 600mg / kg. Another one without medication in mice, as the control group. After administration for 24 hours, the above mice were dissected. Reference Dresden law to take the large intestine 1g, and collect eggs. Take 2ml of each mouse egg suspension in a small test tube incubation, 30min after pipette mouth fluid suspension into the experiment.