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目的:研究生姜提取物6-姜酚对对乙酰氨基酚致小鼠肝脏毒性的保护作用。方法:实验组小鼠在腹腔注射对乙酰氨基酚(900 mg/kg)30 min后,分别给予6-姜酚(30 mg/kg)或标准对照药水飞蓟素(25 mg/kg)。注射对乙酰氨基酚4 h后处死小鼠,检测血清中天冬氨酸氨基转移酶、丙氨酸氨基转移酶、碱性磷酸酶活性,总胆红素的含量及肝匀浆中脂质过氧化及抗氧化情况,如超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽转移酶活性及还原型谷胱甘肽含量。结果:与对照组相比,6-姜酚及水飞蓟素均能显著降低对乙酰氨基酚引起的小鼠血清天冬氨酸氨基转移酶、丙氨酸氨基转移酶、碱性磷酸酶活性及总胆红素含量的升高(P<0.05)。此外,6-姜酚及水飞蓟素有效控制了肝脏内丙二醛的形成及各种抗氧化酶的降低(P<0.05)。结论:本研究的结果证实了6-姜酚具有与水飞蓟素相当的保肝作用。
Objective: To study the protective effect of 6-gingerol extract on liver toxicity induced by acetaminophen in mice. Methods: The mice in the experimental group were given 6-gingerol (30 mg / kg) or standard control silymarin (25 mg / kg) after intraperitoneal injection of acetaminophen (900 mg / kg) for 30 min. Four hours after the injection of acetaminophen, mice were sacrificed and serum aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase activity, total bilirubin and serum lipids were measured Oxidation and anti-oxidation, such as superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione transferase activity and reduced glutathione content. Results: Compared with the control group, 6-gingerol and silymarin significantly reduced the activity of acetaminophen-induced mouse serum aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and total gall Elevated red pigment (P <0.05). In addition, 6-gingerol and silymarin effectively controlled the formation of malondialdehyde in the liver and the decrease of various antioxidant enzymes (P <0.05). Conclusion: The results of this study confirm that 6-gingerol has a comparable hepatoprotective effect to silymarin.