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在表面活性剂CTMAB存在下,维生素E能够定量将Fe(Ⅲ)还原为Fe(Ⅱ)离子,利用显色剂5-硝基-1,10菲咯啉建立了间接分光光度法测定维生素E的方法,在pH 4.6的乙酸-乙酸钠缓冲溶液中,Fe(Ⅱ)-5-硝基-1,10-菲咯啉络合物的最大吸收峰位于502nm处,维生素E的含量在0.3—2.0 mg·L-1范围内符合比耳定律,表观摩尔吸光系数ε502=2.43×104L·mol-1·cm-1。本法用于维生素E片剂、胶丸及注射液中维生素E含量的测定,与药典法对照,2种方法所得结果一致。
In the presence of surfactant CTMAB, vitamin E can quantitatively reduce Fe (Ⅲ) to Fe (Ⅱ) ions, and the indirect spectrophotometric method for the determination of vitamin E by using 5-nitro-1,10-phenanthroline Method, the maximum absorption peak of Fe (II) -5-nitro-1,10-phenanthroline complex is located at 502 nm in acetic acid-sodium acetate buffer solution of pH 4.6, the content of vitamin E is in the range of 0.3-2.0 Beer’s law is obeyed in the range of mg · L-1, and the apparent molar absorptivity ε502 = 2.43 × 104L · mol-1 · cm-1. This method for the determination of vitamin E tablets, capsules and injections of vitamin E content, and pharmacopoeia control, the results of the two methods are consistent.