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目的探讨基因重组质粒pDC316-hIL-24对裸鼠人宫颈癌移植瘤生长和浸润转移的抑制作用,以及其与nm23H1表达变化的关系。方法将18只造模成功的人宫颈癌Hela细胞裸鼠模型随机分为3组,每组6只:BPS缓冲液对照Ⅰ组,空质粒Ⅱ组,IL-24重组质粒Ⅲ组。观察各组裸鼠饮食、体重等一般状况,记录各组裸鼠移植瘤体积、绘制生长曲线。观察裸鼠肿瘤附近淋巴结转移情况,实验结束后根据瘤体重量计算肿瘤抑制率。应用RT-PCR法检测移植瘤IL-24的表达,并用免疫组化法nm23H1蛋白表达变化。结果基因重组质粒pDC316-hIL-24在裸鼠体内转染成功,且有明显的抑瘤效果,抑瘤率为42.9%,与对照组、空质粒组差异有统计学意义(P<0.05)。IL-24重组质粒组肿瘤淋巴结转移较其他组少,差异有显著性(P<0.05)。免疫组化结果显示,IL-24组的转移抑制基因nm23H1蛋白表达明显增多,与其余2组比较差异有统计学意义(P<0.001)。结论重组质粒pDC316-hIL-24能明显抑制宫颈癌裸鼠肿瘤模型的生长,并可能通过上调nm23H1的表达发挥抗肿瘤生长和浸润转移的作用。
Objective To investigate the inhibitory effect of recombinant plasmid pDC316-hIL-24 on the growth, invasion and metastasis of human cervical cancer xenografts in nude mice and its relationship with the expression of nm23H1. Methods Twenty-eight human cervical cancer Hela cells were randomly divided into three groups with 6 mice in each group: BPS buffer control group Ⅰ, empty plasmid Ⅱ group and IL-24 recombinant plasmid group Ⅲ. Observe the general status of diet and body weight of nude mice in each group, record the volume of xenografts in nude mice in each group, and draw the growth curve. Observe the lymph node metastasis in the vicinity of the tumor in nude mice. After the experiment, calculate the tumor inhibition rate according to the tumor weight. The expression of IL-24 was detected by RT-PCR and the expression of nm23H1 protein was detected by immunohistochemistry. Results The recombinant plasmid pDC316-hIL-24 was successfully transfected in nude mice and had a significant anti-tumor effect. The tumor inhibition rate was 42.9%, which was significantly different from that of the control group and empty plasmid group (P <0.05). IL-24 recombinant plasmid tumor-associated lymph node metastasis less than the other groups, the difference was significant (P <0.05). The results of immunohistochemistry showed that the expression of nm23H1 protein was significantly increased in IL-24 group compared with the other two groups (P <0.001). Conclusion The recombinant plasmid pDC316-hIL-24 can significantly inhibit the growth of cervical cancer in nude mice and may play an anti-tumor role in tumor growth and metastasis by up-regulating the expression of nm23H1.