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目的:建立一种简单、快速、灵敏的巴马汀荧光探针法,用于测定抗癌药物奥沙利铂。方法:以奥沙利铂和巴马汀探针对葫芦[7]脲空腔竞争为基础,巴马汀和葫芦[7]脲的水溶液本身无荧光,二者混合后体系荧光显著增强,但当加入无荧光的奥沙利铂后,体系的荧光又显著猝灭。结果:在0.05~1.75μg·m L~(-1)的范围内奥沙利铂的浓度与荧光猝灭值呈良好的线性关系,最低定量限为20 ng·m L~(-1),检测限为6 ng·m L~(-1)。并通过核磁共振和密度泛函理论对其作用机理进行了探究,证实奥沙利铂与葫芦[7]脲可以形成1∶1的稳定包合物。结论:该方法可用于血浆中奥沙利铂的测定。
Objective: To establish a simple, rapid and sensitive palmatine fluorescent probe method for the determination of anti-cancer drug oxaliplatin. METHODS: The aqueous solution of palmatine and gourd [7] urea did not fluoresce on the basis of competition of the urinary gourd [7] urea cavities with oxaliplatin and palmatine probes, and the fluorescence of the system was significantly enhanced after mixing After addition of non-fluorescent oxaliplatin, the fluorescence of the system was significantly quenched. Results: There was a good linear relationship between the concentration of oxaliplatin and the fluorescence quenching value in the range of 0.05 ~ 1.75μg · m L -1 with the lowest limit of quantification being 20 ng · m L -1, The detection limit was 6 ng · m L -1. And its mechanism of action was investigated by NMR and density functional theory, confirming that oxaliplatin and gourd [7] urea can form a stable inclusion complex of 1: 1. Conclusion: This method can be used for the determination of oxaliplatin in plasma.