利用~(15)N标记研究大豆叶面氮素的吸收与分配

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为研究大豆叶面氮素吸收与分配规律,以黑龙江省三江平原大豆主栽品种合丰48为试验材料,采用15N示踪法在大豆R5期进行叶面施氮,研究大豆不同器官对氮素吸收与分配情况。结果表明:不同施氮处理条件下,以4.5kg·hm-2(N3)处理大豆各器官干物质量及氮素积累量显著高于其它处理,其中籽粒干物重23.2 g,总干物重73.9 g,分别较不施氮处理(N0)高7.91%和14.93%;籽粒氮素单株积累量为137.2 g,较不施氮处理(N0)高13.11%。同一施氮水平下,大豆不同器官15N积累量为籽粒>茎>叶>荚皮>叶柄>根,差异达到显著水平。不同施氮处理下,各器官15N积累量随着施氮量的增加而增加,在4.5 kg·hm-2(N3)条件下最高,籽粒单株积累量为8.32 mg。15N在各器官的分配比例与积累量总体趋势一致,不同施氮量间无明显相关性。 In order to study the nitrogen uptake and distribution of soybean leaves, He-48, a soybean cultivar in the Sanjiang Plain of Heilongjiang Province, was used as experimental material to study the effects of different organs of soybean on nitrogen Absorption and distribution. The results showed that under different nitrogen treatments, the dry matter and nitrogen accumulation in organs of soybean increased significantly with 4.5 kg · hm-2 (N3) treatment, including 23.2 g dry matter weight, 73.9 g dry matter weight, Which were 7.91% and 14.93% higher than those of no nitrogen fertilizer (N0), respectively. The accumulative amount of nitrogen per plant was 137.2 g, which was 13.11% higher than that of no nitrogen fertilizer (N0). Under the same nitrogen level, the accumulation of 15N in different organs of soybean was in the order of grain> stem> leaf> peel> petiole> root, the difference reached significant level. Under different nitrogen treatments, the accumulation of 15N in various organs increased with the increase of nitrogen application rate, and reached the highest under the condition of 4.5 kg · hm-2 (N3). The accumulation per plant was 8.32 mg. The distribution trend of 15N in all organs was the same with the general trend of accumulation, and there was no significant correlation between different N rates.
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