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目的探讨硫化氢(H2S)中毒者血中H2S的检测及特征鉴别;方法取新鲜空白血液和5℃下保存1月、1年的血液各2m L,经微波低火加热1min后,吸取顶空气体100μL进行GC/PFPD-S、GC/MS检测;取相同样本进行酸化处理后进行检测;取疑似为H2S中毒者血液直接加热后进行检测。结果新鲜血液和5℃下保存1月、1年的血液均可检出H2S成分,但保存1个月以上的血液可同时检出二硫化碳(CS2)成分;酸化处理后的血液,H2S和CS2成分检出的含量明显比未加酸化的血液高;中毒者血液中除了检出H2S成分外,还检出甲硫醚成分。结论血液样本同时检出H2S和较大量的CS2时,不宜判定为H2S中毒,提示可能为腐败所致,而同时检出H2S和较大量的甲硫醚,则可做出H2S中毒的判断。
Objective To investigate the detection and characterization of H2S in the blood of patients with hydrogen sulfide (H2S) poisoning. Methods Fresh blood samples were collected from fresh blood and stored at 5 ℃ for 1 month and 1 year respectively. After heated for 1 min under microwave heating, Body 100μL for GC / PFPD-S, GC / MS detection; take the same sample for acidification after the test; suspected of being suspected of H2S poisoning blood directly after heating detection. Results Fresh blood and H2S contents were detected in 1 month and 1 year of fresh blood stored at 5 ℃. However, the contents of carbon disulfide (CS2) were also detected in the blood stored for more than 1 month. The acidified blood, H2S and CS2 components The detected content was significantly higher than the non-acidified blood; poisoned blood in addition to detect H2S components, but also detected methyl sulfide components. Conclusions It is not appropriate to determine H2S poisoning when both H2S and CS2 are detected in blood samples, suggesting that it may be caused by corruption. However, simultaneous detection of H2S and larger amounts of dimethyl sulfide may lead to H2S poisoning.