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目的:通过层层自组装技术(LBL)制备内含有脂质体的聚电解质微囊,并对其结构及其对药物的自沉积作用和释放性能进行研究。方法:利用共沉淀法制备碳酸钙模板,在其表面层层组装可生物降解的壳聚糖和海藻酸钠,去除碳酸钙模板后,得到内含脂质体的聚电解质微囊。通过透射电镜(TEM),扫描电镜(SEM)等对微囊的结构、自沉积作用及释放性能进行表征。结果:TEM和SEM显示本实验成功得到了内含脂质体的聚电解质微囊;随着多柔比星给药浓度的增高,载体的囊内药物浓度呈非线性增加,在给药浓度为1 mg.ml-1的条件下,微囊内的最高药物浓度达520.1 mg.ml-1;48 h内,微囊在不同pH(5.0,6.5,7.4)的PBS中的累积释放百分率分别达到59.2%,54.3%,44.8%。结论:内含脂质体的聚电解质微囊具有良好的自沉积作用和释放能力,作为新型的药物载体具有较好的应用前景。
OBJECTIVE: To prepare liposome-containing polyelectrolyte microcapsules by multilayer self-assembly technique (LBL), and to study its structure, drug-induced autodeposition and release properties. Methods: The calcium carbonate template was prepared by co-precipitation method. Biodegradable chitosan and sodium alginate were assembled on the surface layer of the calcium carbonate template. After removing the calcium carbonate template, the liposome-containing polyelectrolyte microcapsules were obtained. The structure, self-deposition and release properties of microcapsules were characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Results: The results of TEM and SEM showed that the liposome-containing polyelectrolyte microcapsules were obtained successfully. With the increase of doxorubicin concentration, the intracapsular drug concentration increased nonlinearly. When the drug concentration was The highest drug concentration in the microcapsules was 520.1 mg.ml-1 under the condition of 1 mg.ml-1. The cumulative release percentage of microcapsules in PBS with different pH (5.0, 6.5, 7.4) within 48 h reached 59.2%, 54.3%, 44.8%. CONCLUSION: Liposome-containing polyelectrolyte microcapsules have good self-deposition and release ability, and have good application prospects as a new type of drug carrier.