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目的:研究黄芪皂苷在体外对人乳腺癌细胞系MCF-7细胞的增殖与凋亡的影响,探讨其抗肿瘤机制。方法:取对数生长期的人乳腺癌细胞MCF-7,分为对照组和不同浓度黄芪皂苷组(2.5μg/m L,5μg/m L,10μg/m L,20μg/m L);采用CCK-8法检测黄芪皂苷对MCF-7细胞增殖抑制率的影响,流式细胞仪检测细胞凋亡情况,Realtime PCR法检测Bcl-2、BAX、MMP-9、nm23-H1 m RNA的表达情况。结果:黄芪皂苷能抑制乳腺癌细胞MCF-7的增殖,抑制作用呈剂量-效应和时间-效应关系;黄芪皂苷能够诱导MCF-7的凋亡,早期凋亡率、晚期凋亡率和总凋亡率均随着黄芪皂苷作用浓度的增加及作用时间的延长而增加;黄芪皂苷能上调BAX和nm23-H1基因的表达,同时使Bcl-2和MMP-9基因的表达下调。结论:黄芪皂苷在体外对乳腺癌细胞MCF-7的增殖和转移有抑制作用,其作用途径是通过调节细胞凋亡相关基因与肿瘤转移相关基因的表达,从而诱导肿瘤细胞的凋亡。
Objective: To study the effects of astragaloside on the proliferation and apoptosis of human breast cancer cell line MCF-7 in vitro and to explore its anti-tumor mechanism. Methods: The human breast cancer cells MCF-7 in logarithmic growth phase were divided into control group and different concentrations of astragaloside group (2.5μg / m L, 5μg / m L, 10μg / m L, 20μg / m L) The effect of astragaloside on proliferation inhibition of MCF-7 cells was examined by CCK-8 assay. The apoptosis of MCF-7 cells was detected by flow cytometry. The expression of Bcl-2, BAX, MMP-9 and nm23-H1 m RNA was detected by Realtime PCR . Results: Astragaloside could inhibit the proliferation of MCF-7 cells in a dose-effect and time-effect manner. Astragaloside could induce apoptosis, early apoptosis, late apoptosis and total apoptosis Astragalus saponins could increase the expression of BAX and nm23-H1 genes, and down-regulate the expression of Bcl-2 and MMP-9 genes at the same time. Conclusion: Astragalus saponins can inhibit the proliferation and metastasis of breast cancer cell MCF-7 in vitro by inducing the apoptosis of tumor cells by regulating the expression of apoptosis-related genes and tumor metastasis-related genes.