中国野生葡萄遗传多样性的RAPD分析

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以起源于中国的18个野生葡萄种(73个株系)、1个欧美杂交种、7个欧洲葡萄品种、1个砧木品种和河岸葡萄(VitisripariaL.)一个品系为试材,利用RAPD技术研究了中国野生葡萄的遗传多样性。从280个随机引物中筛选出20个多态性好的引物扩增供试材料,产生了191条多态性带。应用UPGMA聚类方法(类平均法),获得了83份材料的遗传距离矩阵及聚类分析树系图,且聚为22类12组。河岸葡萄、欧洲葡萄(V.viniferaL.)及欧美杂种与中国野葡萄亲缘关系较远。在中国野葡萄中,菱叶葡萄(V.hancockii Hance)与其他种的亲缘关系最远,秦岭葡萄(V.qinlingensis P.C.He)次之。并可将中国野葡萄资源的18个种、变种和类型分为10组。种内不同花型株系间的遗传变异较大。
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