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建立了利用毛细管电泳简便、准确地测定白头翁汤中黄连和黄柏共煎生物碱煎出量的方法。采用自组装毛细管电泳装置,采用75μmi.d.×50cm弹性石英毛细管,以0 05mol/LNa2B4O7 CH3OH(体积比为85∶15)溶液作缓冲液,运行电压为14kV,检测波长为232nm。另外,通过实验优化了提取溶剂中乙醇的含量。实验结果表明:以小檗碱、巴马汀提取量为指标,30%(体积分数)的乙醇水溶液是提取白头翁汤中黄连和黄柏共煎生物碱的最佳溶剂。小檗碱和巴马汀的质量浓度分别在15 0mg/L～65 0mg/L、12 5mg/L～50 0mg/L时与其峰面积有良好的线性关系;小檗碱的平均回收率不低于95%,巴马汀的平均回收率不低于92%。
Established a capillary electrophoresis using simple and accurate Pulsatilla Decoction Coptis and cork a total of decoction amount of frying method. A self-assembled capillary electrophoresis apparatus was used. A 75 μmi.d × 50 cm flexible quartz capillary tube was used as the buffer solution at a concentration of 0,05 mol / L Na 2 B 4 O 7 CH 3 OH (volume ratio of 85:15). The operating voltage was 14 kV and the detection wavelength was 232 nm. In addition, the ethanol content in the extraction solvent was optimized by experiments. The experimental results show that the extraction of berberine, palmatine as an indicator, 30% (volume fraction) of ethanol aqueous solution is the extraction of Pulsatilla Coptis and Phellodendron common alkaloids the best solvent. The concentration of berberine and palmatine in the concentration range of 15 0mg / L ~ 65 0mg / L, 125mg / L ~ 50 0mg / L had a good linear relationship with the peak area; the average recovery of berberine was not low At 95%, palmatine average recovery of not less than 92%.