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大肠杆菌为引起小儿腹泻的重要病因。本文首次报导了人乳IgA抗大肠杆菌热不稳定毒素(LT)抗体对保护母乳喂养儿免患LT所致胃肠炎的作用。在危地马拉边远地区任取130名0～8个月婴儿为调查对象。每周1次家庭访视,发现腹泻者每2～3周取1次大便标本作微量生化检查,如系母乳喂养儿同时采集奶标本送检。大肠杆菌的检测将置于Cary-Blair转种培养基的大便,涂划在志贺、沙门菌和McConkey琼脂平板上,经24小时孵育,挑取乳糖发酵菌落,经生化鉴定确认为大肠杆菌,再吸取酪蛋白氨基酸肉汤汁,通过鼠肾上腺肿瘤(Y-1)细胞分析以确定LT的
Escherichia coli is an important cause of diarrhea in children. This paper reports for the first time the role of human milk IgA anti-E.coli thermotolerant toxin (LT) antibodies in protecting breast-fed infants against LT-induced gastroenteritis. In the remote Guatemala region, 130 0-8 month-old infants were selected as the survey subjects. Weekly home visit and found diarrhea every 2 to 3 weeks to take a stool specimen for microbiochemical examination, such as the Department of Breast milk while collecting milk samples for inspection. Escherichia coli test will be placed in Cary-Blair transformant stool, painted in Shiga, Salmonella and McConkey agar plates, incubated for 24 hours to pick the lactose fermentation colonies confirmed by biochemical identification of E. coli, Casein amino acid broth was again pipetted and analyzed by murine adrenal tumor (Y-1) cells to determine the effect of LT