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1968年瑞典细胞化学家Caspersson首先提出了动物染色体萤光分带技术,即Q带。1969年他又成功地把Q带技术用于植物—蚕豆,从而染色体的带型分析补充了组型分析的不足,为细胞学、细胞遗传学、染色体工程等方面的研究,开拓了新景。1970年Pardue和Gall发现染色体的着丝点附近分布着最初构成的异染色质与Giemsa染剂能发生特异的分带染色即G带。1972年Vosa等把G带技术用于研究植物染色体。1972年Sumner创用BSG法植物染色体分带技术(先用碱—Ba(OH)_2,再用盐—柠檬酸钠和氯化钠即SSC溶液处理细胞,
1968 Swedish cell chemist Caspersson first proposed the zoning of animal chromosome fluorescence banding. In 1969, he successfully applied the Q-banding technique to plants-fava beans. Thus, the chromosome-based banding analysis supplemented the lack of profiling and opened up new prospects for the study of cytology, cytogenetics and chromosome engineering. In 1970, Pardue and Gall discovered that the heterochromatin, which originally formed in the vicinity of the centromere of a chromosome, interacts with Giemsa stain specifically for G banding. In 1972, Vosa et al. Used G-band technology to study plant chromosomes. In 1972, Sumner created the plant chromosome partitioning technique of BSG (using alkali-Ba (OH) 2 first, followed by salt-sodium citrate and sodium chloride (SSC)