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目的 了解我国肾综合征出血热疫苗生产毒株Ｌ９９株的分子特征及抗原性的分子基础。方法 设计出不同的引物，用ＰＣＲ方法提取细胞总ＲＮＡ，逆转录扩增产物纯化后克隆Ｔ载体，纯化后测序，序列用ＤＮＡＳ ＴＡＲ软件拼接，并对其序列进行分析。结果Ｌ９９株全基因组由Ｌ片段６５３３个，Ｍ３６５２个，Ｓ１７６４个核苷酸组成， 分别编码２１５１、１１３３、４２９个氨基酸，Ｌ９９与同型病毒间同源率高达９５．５％－９９．４％。结论 为研究疫苗毒株的生物 学特性、致病机理等提供了分子基础。
Objective To understand the molecular characteristics and antigenicity of L99 strain of Chinese hemorrhagic fever with renal syndrome vaccine. Methods Different primers were designed and the total RNA was extracted by PCR. The T vector was cloned by reverse transcription amplification, purified and sequenced. The sequences were analyzed by DNAS TAR software. Results The whole genome of L99 strain consisted of 6533 L fragments, M3652 and S1764 nucleotides encoding 2151, 1133 and 429 amino acids, respectively. The homology between L99 and homologous viruses was as high as 95.5% -99.4%. Conclusions The molecular basis for the study of the biological characteristics of vaccine strains, pathogenesis and so on.