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采用响应面法优化附子甘草药对中总生物碱的回流提取工艺。以总生物碱提取率为评价指标,通过单因素试验考察了浸泡时间、甘草质量、液料比、回流时间、回流次数等因素对总生物碱提取率的影响。在单因素试验的基础上,利用响应面设计优化试验。回流提取的最优工艺条件为:回流时间为120 min;m(附子)∶m(甘草)为1∶1.15;液料比为30。验证试验表明,拟合得到的回归模型与实际符合较好。
Response surface methodology was used to optimize the reflux extraction process of total alkaloids from Aconite licorice. The extraction rate of total alkaloids was taken as the evaluation index. The effects of soaking time, liquorice ratio, liquid-to-liquid ratio, reflux time and reflux times on the extraction rate of total alkaloids were investigated by single factor experiments. Based on the single factor experiment, the response surface design is used to optimize the experiment. The optimum conditions of reflux extraction were as follows: reflux time was 120 min; m (Aconitum): m (licorice) was 1: 1.15; Verification tests show that the fitting regression model is in good agreement with the actual situation.