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在扫描电镜下对见在葡萄果粒表面蜡质层下有一种形状无规则的小孔,其直径约为0.5—2um。用萤光素钠处理果面在紫外分析仪下可看出小孔的密集程度。我们研究了31个品种果皮小孔密度和感染葡萄炭疽病[Glqmerella cjngulafa(Ston)Spauld efSehvenk]的关系,发现二者呈显著直线正相关。并看出欧亚种(V·vinifera L)品种的小孔密度较大,较易感病;欧美杂种(V·vinifera L·V·labrusca L),欧山杂种(V·vinifera L·V·anurensis Rupr)和法国杂种(Seibel和Seyve—Villard系)品种的小孔密度较小也较抗病。根据小孔和病情的回归相关,种和种间的差异以及多年的田间病情观察,初步认为小孔密度指数20％以下,不超过25％为鉴定抗炭疽病品种或杂种后代的一项指标。
Under the scanning electron microscope to see in the grapes under the surface of the wax layer has a random shape of small holes, the diameter of about 0.5-2um. Treating the fruit surface with sodium luciferin The density of the pores can be seen under the UV analyzer. We studied the relationship between the stoma density of 31 cultivars and the infection of grape anthracnose [Glqmerella cjngulafa (Ston) Spauld ef Sevenk], and found a significant linear positive correlation between them. The results showed that V. vinifera L. was more susceptible to pinhole density. V. vinifera L.V. labrusca L, V. vinifera L.V. Anurensis Rupr and French hybrids (Seibel and Seyve-Villard lines) were smaller and more resistant to disease. According to the correlation between the small hole and the regression of the disease, the difference of the species and the interspecies and the observation of the disease in the field for many years, it is preliminarily thought that the index of stomatal density below 20% and not more than 25% is an index to identify the anti-anthracnose variety or hybrid offspring.