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目的:探讨恶唑酮诱导小鼠溃疡性结肠炎(UC)模型的建立,并评估其在UC研究中的价值。方法:30只BALB/C小鼠被随机分成模型组、对照组和治疗组,每组10只。予恶唑酮建模后,治疗组给予柳氮磺胺吡啶灌胃治疗,观察三组小鼠的疾病活动指数(DAI),一周后处死所有小鼠,取其病变结肠观察组织学改变,并检测脾组织重量,采用酶联免疫吸附法(ELISA)测定三组小鼠血清白细胞介素IL-10、IL-2的含量。结果:模型组小鼠病变结肠组织学改变与人类UC病变结肠形态相似,其DAI和组织学损伤评分均较对照组有明显改变,血清IL-10含量显著增高,IL-2含量明显下降,免疫脏器脾脏的重量明显减轻。结论:恶唑酮能诱导出与人类UC发病相似的实验小鼠模型,可作为研究UC病因、发病机制和药物治疗较为理想的模型。
Objective: To investigate the establishment of oxazolone-induced ulcerative colitis (UC) model in mice and to evaluate its value in UC. Methods: Thirty BALB / C mice were randomly divided into model group, control group and treatment group, with 10 rats in each group. After the oxazolone model was established, the treatment group was treated with sulfasalazine gavage. The disease activity index (DAI) of the three groups of mice was observed. All the mice were sacrificed one week later. The histological changes of the mice were observed and the histological changes were observed. Spleen tissue weight was measured by enzyme-linked immunosorbent assay (ELISA) of three groups of mice serum interleukin IL-10, IL-2 content. Results: The pathological changes of colonic mucosa in model mice were similar to those of human colonic mucosa. The DAI and histological damage scores of the model group were significantly changed compared with those of the control group. The levels of IL-10 and IL-2 in the model group were significantly decreased The weight of organs spleen significantly reduced. Conclusion: Oxazolone can induce experimental mouse model similar to that of human UC. It can be used as a model to study the etiology, pathogenesis and drug therapy of UC.